WebThis model has been discontinued. GALLERY. × WebRéférence CD601-02. Conditionnement : 10x100µl. Marque : TransGen Biotech. Manual. Product Details. BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10 7 cfu/μg DNA (tested by pUC19 plasmid DNA).
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WebApr 18, 2024 · Cell culture. The HEK 293T cell line was cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco, C11885500BT) containing 10% fetal bovine serum (FBS) (Gibco, 16000-044) and 1× Penicillin-Streptomycin Solution (Corning, 30-002-CI) at 37°C in an atmosphere comprising 5% CO 2.. E. coli Trans1-T1 (Transgene Biotech., CD501-03) … WebMar 26, 2024 · transformed into E.coli T rans BL21 (DE3) (CD601-02, T ransGen Biotech), which were grow n . 126. at 37℃ in LB broth containing 0.1 mg/ml of Kanamycin until the OD600 reached 0.4-0.6. 127. microsoft streets and trips alternative free
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WebOct 29, 2024 · # CD601-02) to obtain the N-terminally acetylated α-syn protein 40. Cells were harvested after protein expression at 37 °C for 4 h with 1 mM isopropyl-1-thio-d g for 25 min. Then the supernatant was processed by boiling at 100 °C for 10 min, streptomycin treatment (20 mg/ml), pH adjustment to 3.5 using 2 M HCl, and dialysis 50 mM Tris–HCl ... WebMay 26, 2024 · Amyloid aggregation of α-synuclein (α-syn) is closely associated with Parkinson’s disease (PD) and other synucleinopathies. Several single amino-acid mutations (e.g. E46K) of α-syn have been ... Webtransformed into E.coli Trans BL21(DE3) (CD601-02, TransGen Biotech), which were grown at 37°C in LB broth containing 0.1 mg/mL kanamycin until the OD 600 reached 0.4–0.6. Isopropyl-β-D- microsoft strengths and weaknesses