Phospho mtor ser2448

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August undefined, 2024

WebMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3 … WebmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by …

PathScan® Phospho-mTOR (Ser2448) Sandwich ELISA Kit

WebPhospho-mTOR (Ser2448) (49F9) Rabbit mAb (BSA and Azide Free) detects endogenous levels of mTOR protein only when phosphorylated at Ser2448. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human … WebJan 9, 2024 · Both mTORC1 and mTORC2 are regulated by phosphorylation, complex formation and localization. So far, the role of p70S6K-mediated mTOR S2448 phosphorylation has not been investigated in detail. Here, we report that endogenous mTOR phosphorylated at S2448 binds to both, raptor and rictor. roasted cherry tomato sauce freeze

Phospho mTOR (Ser2448) cellular HTRF kit Cisbio

WebInvitrogen Anti-Phospho-mTOR (Ser2448) Monoclonal (MRRBY), eBioscience™, Catalog # 12-9718-42. Tested in Flow Cytometry (Flow) applications. This antibody reacts with … WebWe show that Ser-2448 is directly phosphorylated byp70S6 kinase in vitro and that expression of a rapamycin-resistantp70S6 kinase in cells maintains Ser-2448 phosphorylation in the presence ofrapamycin. Furthermore, changes in amino acid availability, which affectsp70S6 kinase activity, also modulates Ser-2448 phosphorylation. roasted cherry tomatoes with goat cheese

Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb #5536

phosphorylation-independent mtorc1 inhibition by the ... - 豆丁网

WebPhospho-mTOR (S2448) and Total mTOR ELISA Kit (ab279869) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated mTOR protein in your experimental model system, you can verify pathway activation in your cell lysates. WebThe MSD Phospho(Ser2448)/Total mTOR Assay is available on 96-well 4-Spot plates. This datasheet outlines the performance of the assay. Typical Data Representative results for … roasted chestnut coffeeWebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Validated for Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, … snoop dogg clint eastwood

"WebProduct Description. The PathScan ® Phospho-mTOR (Ser2448) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects … " - Phospho mtor ser2448

Phospho mtor ser2448

WebMonoclonal Antibody for studying mTOR (Ser2448) phosphate. Cited in 1811 publications. Validated for WB, IP, IF. Available in 2 sizes. Highly … Web斯氏毛滴虫pcr检测试剂盒厂家上海联祖生物相关产品:28s rrnp检测试剂盒实验流程1.实验前标准品、试剂及样本的准...

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WebmTOR抑制剂的筛选方法. 分析：OD值越小，mTOR的Ser2448磷酸化程度越低，抑制剂作用越强。. （2）荧光共振能量转移（FRET）. 荧光共振能量转移是指两个荧光发色基团在足够靠近（7~10nm）时，当供体分子吸收一定频率的光子后被激发到更高的电子能态，在该电子回 … WebPrinciple of Assay. This mTOR (phospho Ser2448) Cell Based ELISA Kit allows for the detection of mTOR (phospho Ser2448) and the effects that certain stimulation conditions have on mTOR (phospho Ser2448) expression in different cell lines. Qualitative determination of mTOR (phospho Ser2448) concentration is achieved by an indirect …

WebJan 15, 2009 · mTOR phosphorylated at S2448 can be found in a complex with both, raptor and rictor. Total lysates of logarithmically growing HEK293 cells were prepared and used for immunoprecipitation of endogenous raptor or rictor. WebPrimary antibodies for immunohistochemistry (IHC) included anti-mTOR, anti-phospho-mTOR Ser2448, anti-S6 ribosomal protein, anti-Beclin1 (all from Abcam, Cambridge, UK) …

WebAnti-phospho-mTOR (Ser2448) Antibody Upstate®, from rabbit; Synonyms: FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1; find … WebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, which indicates the mTORC1...

WebInvitrogen Anti-Phospho-mTOR (Ser2448) Polyclonal, Catalog # 44-1125G. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts …

WebThe mTOR subunit of mTORC1 and 2 is phosphorylated at Ser2448 in a PI3/Akt- signaling-dependent manner; this post-translational modification is associated with increased … snoop dogg clothes and shoesWebresults in elevated mTOR activity in vitro and in cells (18, 20). In this study, we demonstrate that mTOR phosphorylation at Ser-2448 is blocked by rapamycin, and this effect is inde-pendent of the AKT activation status, which suggests that Ser-2448 phosphorylation is catalyzed by a protein kinase other than AKT. We show that Ser-2448 is ... snoop dogg christian conversionWebThe Phospho-mTOR (Ser2448) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2448) Antibody has been validated for the following applications: Western Blot. Phospho-mTOR (Ser2448) Antibody detects … snoop dogg commentary boxing mike tysonWebApr 5, 2024 · Several upstream signals, including ERK, converge on the phosphorylation and inhibition of Tuberous Sclerosis Complex (TSC) to activate mTORC1 [20, 21]. mTOR functions as the catalytic subunit of two individual complexes, known as … snoop dogg clothing line websiteWebFeb 12, 2024 · We measured the levels of mTOR phosphorylation at Ser2448, and the mTOR target protein 49, the ribosomal protein S6 kinase (p70S6K) phosphorylation at Thr389, … roasted cherry tomato pastaWebPhospho-mTOR (Ser2448) Antibody (25-9718-42) in Flow Normal human peripheral blood cells were unstimulated (left) or stimulated with Anti-Human CD3 and CD28 Functional … roasted cherry tomatoes with balsamic vinegarWebmTOR (phospho Ser2448) stained by mTOR (phospho Ser2448) antibody (GTX132803) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min GTX132803 WB Image Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody … roasted chestnuts amazon