WebJul 27, 2024 · Schematic organization of antibody-dependent THP-1 cell-mediated phagocytosis assay. (a)The dihydroethidium-stained P. falciparum IEs were added to … WebFigure 1. ADCP assay using FcgRIIa expressing reporter cell line. Modified Jurkat cell line expressing FcgRIIa were co-cultured with target cells in the presence of a test article (4) …
THP-1 requires differentiation before Phagocytosis assay?
WebNov 17, 2024 · We have implemented an improved, cost-effective, and highly reproducible protocol for a simple and rapid differentiation of the human leukemia monocytic cell line … WebHuman monocytic cell line THP-1 was purchased from cell bank of Chinese Academy of Sciences (Shanghai, ... Phagocytosis assays. After M2-polarized macrophage cells treated with Terb at different concentration ... 10 or 15 min were the second group. The following procedures were performed according to the manufacturer’s protocol. south shore cyclery cudahy
MAINTENANCE CULTURE OF THP-1 CELLS - Bowdish
WebJan 10, 2024 · THP-1 cells were seeded in 2-well chamber slides, differentiated and treated as indicated. Macrophages were then assessed for phagocytic activity using the … Web1 day ago · The study was conducted under 150 approved ethics protocols with written ... . 170 In vitro macrophage phagocytosis of ITP serum-opsonized platelets 171 Human macrophage phagocytosis assays were performed as ... X-axis, log10 fluorescence intensity. (B) THP-1-CD16A 634 macrophage phagocytosis of platelets opsonized with anti ... WebJan 8, 2024 · The protocol was as for LP2 with the exception that butan-1-ol was replaced with 0.65 mL pentanol. Synthesis protocol of SNP01. Absolute ethanol ... In all … teak construction llc